Analysis of Autoantibodies to 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase Using Different Technologies
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چکیده
منابع مشابه
Analysis of Autoantibodies to 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase Using Different Technologies
Diagnostic tests are needed to aid in the diagnosis of necrotizing myopathies associated with statin use. This study aimed to compare different technologies for the detection of anti-HMGCR antibodies and analyze the clinical phenotype and autoantibody profile of the patients. Twenty samples from myositis patients positive for anti-HMGCR antibodies using a research addressable laser bead assay a...
متن کاملPurification of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
The enzyme 3-hydroxy-3-methylglutaryl coenzyme agarose, glucose-6-phosphate dehydrogenase, and aldolA reductase from human liver was purified to homogeneity. ase were from Sigma Chemical co.; agarose-hexaneHMG-CoA was from P-L Biochemicals; bovine serum Electrophoresis of the purified human enzyme on non-denaturing gels revealed a single protein-staining band that co-migrated with reductase act...
متن کاملStatins and Myotoxic Effects Associated With Anti-3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Autoantibodies
Statins have a variety of myotoxic effects and can trigger the development of inflammatory myopathies or myasthenia gravis (MG) mediated by immunomodulatory properties. Autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) have been identified in patients with statin-associated myopathy. The purpose of the present study is to develop an enzyme-linked immunosorbent assay (ELI...
متن کاملAutoantibodies against 3-hydroxy-3-methylglutaryl-coenzyme A reductase in patients with statin-associated autoimmune myopathy.
OBJECTIVE In addition to inducing a self-limited myopathy, statin use is associated with an immune-mediated necrotizing myopathy (IMNM), with autoantibodies that recognize ∼200-kd and ∼100-kd autoantigens. The purpose of this study was to identify these molecules to help clarify the disease mechanism and facilitate diagnosis. METHODS The effect of statin treatment on autoantigen expression wa...
متن کاملChrysosporin, a new inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase produced by Chrysosporium pannorum.
Cholesterol is synthesized via more than 20 enzymatic reactions. This pathway is mainly regulated by the enzyme, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA)reductase1}. Recent clinical results have shown that HMG-CoA reductase inhibitors such as lovastatin2) (monacolin K3)) are safe and effective drugs for treatment of hypercholesterolemia4). Wehave previously reported that Chrysosporium pa...
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ژورنال
عنوان ژورنال: Journal of Immunology Research
سال: 2014
ISSN: 2314-8861,2314-7156
DOI: 10.1155/2014/405956